Composite

Part:BBa_K3453160

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-04)


Rosewood DmTrnL-UAA Trigger (Full) transcription unit

This part is a transcription unit of the Rosewood DmTrnL-UAA Trigger (Full) (BBa_K3453060) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA thereby exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).

T--Evry Paris-Saclay--toehold-switch.png

Figure 1. Toehold switches principle.

This part is a transcription unit of BBa_K3453060 which is a trigger for the 6 toehold switches we designed for sequence-based detection of rosewood (BBa_K3453051, BBa_K3453052, BBa_K3453053, BBa_K3453054, BBa_K3453055, BBa_K3453056).

This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.

Full results are available on the BBa_K3453151, BBa_K3453152, BBa_K3453153, BBa_K3453154, BBa_K3453155, BBa_K3453156 pages in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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